Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108

Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation

Cristian Forestan^1¹  Silvia Farinati^1²  Jacques Rouster^2³ 

[1] Department of Agronomy Food Natural Resources, Animals and Environment (DAFNAE) Agripolis, University of Padova, 35020 Legnaro (PD), Italy^1;GM Trait Cereals, Biogemma, Centre de Research de Chappes, 63720 Chappes, France^2;The Institute of Agricultural Biology and Biotechnology (IBBA), Consiglio Nazionale delle Ricerche (CNR), 20133 Milano, Italy^3

关键词: histone acetylation/deacetylation;    histone deacetylases;    HDA108;    transcriptomic analysis;    Zea mays;   

DOI  :  10.1534/genetics.117.300625

学科分类：医学（综合）

来源: Genetics Society of America

【 摘 要 】

Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated histone tails that consequently interact more closely with DNA, leading to chromatin state refractory to transcription. Zea mays HDA108 belongs to the Rpd3/HDA1 HDAC family and is ubiquitously expressed during development. The newly isolated hda108/hda108 insertional mutant exhibited many developmental defects: significant reduction in plant height, alterations of shoot and leaf development, and alterations of inflorescence patterning and fertility. Western blot analyses and immunolocalization experiments revealed an evident increase in histone acetylation, accompanied by a marked reduction in H3K9 dimethylation, in mutant nuclei. The DNA methylation status, in the CHG sequence context, and the transcript level of ribosomal sequences were also affected in hda108 mutants, while enrichment in H3 and H4 acetylation characterizes both repetitive and nonrepetitive transcriptional up-regulated loci. RNA-Seq of both young leaf and anthers indicated that transcription factor expression is highly affected and that the pollen developmental program is disrupted in hda108 mutants. Crosses between hda108/hda108 and epiregulator mutants did not produce any double mutant progeny indicating possible genetic interactions of HDA108 with distinct epigenetic pathways. Our findings indicate that HDA108 is directly involved in regulation of maize development, fertility, and epigenetic regulation of genome activity.

【 授权许可】

CC BY