Restoration of immune suppressor function of regulatory B cells collected from patients with allergic rhinitis with Chinese medical formula Yupingfeng San

American Journal of Translational Research

Fei Ma¹  Li-Juan Song²  Cai-Jie Zhou³  Wen-Jing Liao⁴ 

[1] Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China;Longgang ENT Hospital, Shenzhen ENT Institute, Shenzhen, China;Longgang Traditional Chinese Medicine Hospital and Beijing University of Chinese Medicine Shenzhen Hospital, Shenzhen, China;Research Center of Allergy and Immunology, School of Medicine, Shenzhen University, Shenzhen, China

关键词: Allergy;    immune regulation;    B cell;    Bcl2L12;    Chinese medicine;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Immune dysregulation, such as defects in immune suppressor function, plays an important role in the pathogenesis of many immune disorders including allergic rhinitis (AR). Some Chinese traditional medical formulae have an immune regulatory function. This study aims to restore the immune suppressor function in regulatory B cells (Bregs) collected from AR patients with a Chinese medical formula, Yupingfeng San (YPFS). In this study, Bregs were isolated from blood samples collected from AR patients and healthy (HA) subjects. The capacity of Breg in suppressing effector T cell (Teff) proliferation was observed in an in vitro experiment to be used as an indicator of immune suppressor function of Breg. The effects of YPFS on promoting Bregs’ immune suppressor functions were tested in a cell culture study. The results showed that the number of peripheral Breg in AR patients was not significantly different from that in HA subjects, while the immune suppressor function of AR Breg was compromised. Bcl2L12 expression was higher in AR Bregs than that in HA Bregs. A negative correlation was identified between expression of Bcl2L12 and IL-10 in AR Bregs. Exposure of AR Bregs to YPFS in the culture suppressed the expression of Bcl2L12 and improved their immune suppressor function. In conclusion, YPFS can restore the immune suppressor function of AR Bregs via inhibiting the expression of Bcl2L12. The data suggest that YPFS has the potential to be used in the improvement of immune dysfunction, such as AR.

【 授权许可】

CC BY-NC