Characterization and expression of Xiphophorus maculatus microsatellite Msb069 full sequence in subgenus Poecilia

Journal of genetics

KEONG BUN POH^1¹ 

[1] Faculty of Science and Mathematics, Department of Biology, Universiti Pendidikan Sultan Idris, 35900, Tanjong Malim, Perak, Malaysia^1

关键词: microsatellite;    Msb069 full sequence;    quantitative trait loci;    dorsal fin length;    Poecilia;    Xiphophorus maculatus;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Msb069 primer pairs encompassed region is believed to be associated with a quantitative trait loci (QTL) of dorsal fin length in subgenus Poecilia. However, detailed investigation on Msb069 which originated from Xiphophorus on subgenus Poecilia remains unexplored. In this study, full sequence of Msb069 was characterized by sequencing bioinformatics analysis and gene expression. The sequence analysis of Msb069 primer pairs encompassed region on three species of Poecilia revealed higher number of microsatellite tandem repeats in Poecilia latipinna (ATG16) compared to P. sphenops (ATG13–14). There is no notable pattern of ATGtandem repeats discovered in the hybrids. The full sequence of Msb069 is 734 bp in length and showed a 233 bp conserved region between Xiphophorus and Poecilia. BLAST search performed on this sequence revealed no significant similarities. Nonquantitative RT-PCR exhibited the presence of Msb069 transcripts in three different tissues in subgenus Poecilia. Meanwhile, quantitative RTPCR expression on two different tissues showed relatively higher expression of Msb069 transcript in P. latipinna dorsal fin tissues inboth male and female fishes, suggesting a repressive function of this transcript with respect to dorsal fin length. However the exact gene expression event of Msb069 is still unknown and requires further investigation.

【 授权许可】

CC BY