METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS

Quimica nova

Costa, José Luiz da¹  Cruz, Pedro L. R. da²  Canuto, Gisele A. B.³ 

[1] Universidade Estadual de Campinas, Campinas, Brazil;Universidade Federal de São Paulo, Diadema, Brazil;Universidade de São Paulo, São Paulo, Brazil

关键词: metabolomics;    untargeted metabolomics;    targeted metabolomics;    metabolomics workflow.;   

DOI  :  10.21577/0100-4042.20170134

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.

【 授权许可】

CC BY