Revista de microbiologia | |
LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis | |
Trangoni, Marcos D.1  | |
[1] National Agricultural Technology Institute, Buenos Aires, Argentina | |
关键词: loop-mediated isothermal amplification; molecular typing; brucellosis; paratuberculosis.; | |
DOI : 10.1590/S1517-838246220131206 | |
学科分类:农业科学(综合) | |
来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology | |
【 摘 要 】
In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols were suitable for specifically identifying the respective pathogens; in the case of Brucella, it also allowed the identification of all the biovars tested. We conclude that LAMP is a suitable rapid molecular typing tool that could help to shorten the time required to identify insidious bacteria in low-complexity laboratories, mainly in developing countries.
【 授权许可】
CC BY-NC
【 预 览 】
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RO201910259514102ZK.pdf | 1496KB | download |