Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells - ScienceDirect

Stem Cell Research

Sameza Naseer^1¹  Elaine L. Ferguson^1,2² 

[1] Cardiff Institute of Tissue Engineering and Repair, Cardiff University, 10 Museum Place, Cardiff, South Glamorgan, CF10 3BG, UK^2;Oral and Biomedical Sciences, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XY, UK^1

关键词: BCA;    bicinchoninic acid;    bFGF;    basic fibroblast growth factor (FGF-2);    CLSM;    confocal laser scanning microscopy;    DMAP;    4-dimethylaminopyridine;    DMF;    N,N-dimethyl formamide;    DMSO;    dimethyl sulfoxide;    DNA;    deoxyribonucleic acid;    EDC;    1-ethyl-3-(3-dimethylaminopropyl carbodiimide hydrochloride);    EGF;    epidermal growth factor;    EMEM;    eagle';    s minimum essential media;    FCS;    fetal calf serum;    FPLC;    fast protein liquid chromatography;    GF;    growth factor;    GFAP;    glial fibrillary acidic protein;    GPC;    gel permeation chromatography;    HAMC;    hyaluronan and methyl cellulose;    Hep2;    human epidermoid carcinoma;    MAP2;    microtubule-associated protein 2;    mNSC;    mouse neural stem cell;    MTT;    3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;    NSC;    neural stem cell;    Olig2;    oligodendrocyte transcription factor;    PBS;    phosphate buffered saline;    PLGA;    poly(lactic-co-glycolic acid);    SCI;    spinal cord injury;    sulfo-NHS;    N-hydroxysulfosuccinimide;    TUNEL;    terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labelling;    Polymer therapeutics;    Biodegradable polymers;    Growth factors;    Neural stem cells;    Controlled release;   

DOI  :  10.1016/j.scr.2018.10.008

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

An essential aspect of stem cell in vitro culture and in vivo therapy is achieving sustained levels of growth factors to support stem cell survival and expansion, while maintaining their multipotency and differentiation potential. This study investigated the ability of dextrin (~74,000 g/mol; 27.8 mol% succinoylation) conjugated to epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF; or FGF-2) (3.9 and 6.7% w/w protein loading, respectively) to support the expansion and differentiation of stem cells in vitro via sustained, controllable growth factor release. Supplementation of mouse neural stem cells (mNSCs) with dextrin-growth factor conjugates led to greater and prolonged proliferation compared to unbound EGF/bFGF controls, with no detectable apoptosis after 7 days of treatment. Immunocytochemical detection of neural precursor (nestin) and differentiation (Olig2, MAP2, GFAP) markers verified that controlled release of dextrin-conjugated growth factors preserves stem cell properties of mNSCs for up to 7 days. These results show the potential of dextrin-growth factor conjugates for localized delivery of bioactive therapeutic agents to support stem cell expansion and differentiation, and as an adjunct to direct neuronal repair.

【 授权许可】

Unknown