Pericentrin expression in pancreatic β cells is associated impaired glucose tolerance

American Journal of Translational Research

Yanping Gong¹  Liangchen Wang²  Chunlin Li³ 

[1] Department of Endocrinology, Chinese Air Force General Hospital of Peoples Liberation Army, Beijing 100142, China;Department of Geriatric Endocrinology, Chinese Peoples Liberation Army General Hospital, National Clinical Research Center for Geriatric Disease, Beijing 100853, China;Department of Nephrology, Chinese Peoples Liberation Army General Hospital, Chinese Peoples Liberation Army Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China

关键词: Pericentrin;    first phase;    insulin secretion;    impaired glucose tolerance;    prevention;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Objective: To explore the role and mechanism of pericentrin (PCNT) in impaired glucose tolerance. Methods: Mouse model of specific PCNT reduction in β-cells (PCNTβPCNT) was built using a Tet-on induction system; mouse model of impaired glucose tolerance was built by high-fat feeding. MIN6 cells were divided into control and Si-PCNT groups. Results: An obvious decrease in PCNT, F-actin, and insulin expression in Si-PCNT cells (P < 0.01) was observed, and the stimulating effect of GLP-1 on first phase insulin secretion disappeared in Si-PCNT cells. PCNTβ exhibited impaired first phase insulin secretion and abnormal glucose tolerance (P < 0.05 or P < 0.01). Fewer insulin granules smaller than 300 nm were detected in PCNTβ (P < 0.05). PCNT expression decreased progressively with insulin resistance (P < 0.05 and P < 0.01). First phase insulin secretion and glucose tolerance decreased with PCNT levels. The homeostasis model assessment-insulin resistance was negatively correlated with PCNT expression. Conclusions: PCNT plays an important role in modulating first phase insulin release by adjusting distribution of insulin granules and was closely related to development of impaired glucose tolerance induced by the high-fat diet. PCNT might be a therapeutic target for diabetes prevention.

【 授权许可】

CC BY-NC