Tracing the Origin of the Anomalous Matrix Suppression in Matrix‐assisted Laser Desorption Ionization and Extending the Linear Dynamic Range During Analyte Quantification

Bulletin of the Korean Chemical Society

Taeghwan Hyeon¹  Hyo In Song²  Myung Soo Kim³ 

[1] Applied Surface Technology Ascend (ASTA) Suwonsi 07675 Korea;Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 08826 Korea;Disease Target Structure Research Center Korean Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 34141 Korea

关键词: Matrix‐;    assisted laser desorption ionization quantification;    Linear dynamic range;    Matrix suppression;    Detector saturation;    Liquid matrix;   

DOI  :  10.1002/bkcs.11368

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

In the matrix‐assisted laser desorption ionization‐based quantification method developed in this laboratory, a linear calibration curve is constructed by plotting the analyte‐to‐matrix ion abundance ratio as a function of the analyte concentration. As the analyte concentration increases, however, the curve deviates from linearity. Previously, we observed that the occurrence of this deviation correlated with the decrease in the matrix ion signal, or matrix suppression. In this work, we found that saturation of the detector was one of the factors responsible for the above phenomena. As we decreased the detector gain, the upper limit of the linear dynamic range increased by as much as one order of magnitude. Even after a substantial decrease in the detector gain, however, the nonlinearity remained at a high analyte concentration, which we cannot account for at present. The method developed in this work can be useful for the quantitative imaging of analytes in heavily contaminated samples.

【 授权许可】

Unknown