Investigating the transmissibility of tet(W) in bifidobacteria exposed to acid and bile stress

Bioscience of Microbiota, Food and Health

Agnieszka POLIT¹ 

[1] School of Life Sciences, Faculty of Health and Life Sciences, Coventry University, Priory Street, Coventry CV1 5FB, United Kingdom

关键词: Bifidobacterium;    gastrointestinal stress;    probiotics;    tet(W);    antimicrobial resistance;   

DOI  :  10.12938/bmfh.17-017

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

Transfer of antibiotic resistance genes from probiotic bacteria to pathogens poses a safety concern. Orally administered probiotics are exposed to stressful conditions during gastrointestinal transit. In this study, filter mating experiments were performed to investigate the potential role of exposure of Bifidobacterium isolates to acid and bile stress on the transfer of a tetracycline resistance gene, tet(W), to Enterococcus faecalis ATCC 51299. No E. faecalis transconjugants were obtained after mating with either stressed or unstressed Bifidobacterium, thereby suggesting that tet(W) could not be transferred as a result of exposure to gastrointestinal stresses.

【 授权许可】

CC BY