The variation profile of intestinal microbiota in blunt snout bream ( Megalobrama amblycephala ) during feeding habit transition

BMC Microbiology

Han Liu¹  Jin Wei²  Xianwu Guo³ 

[1] College of Fisheries, Huazhong Agricultural University, Wuhan, Peoples Republic of China;College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China;Lab of Biotecnologa Genmica, Centro de Biotecnologa Genmica, Instituto de Politcnico Nacional, Reynosa, Mexico

关键词: Blunt snout bream;    Gut microbiota;    Feeding habit transition;    Core microbiome;    Enzyme activity;    Cellulose-degrading bacteria;   

DOI  :  10.1186/s12866-018-1246-0

学科分类：微生物学和免疫学

来源: BioMed Central

【 摘 要 】

The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development. Gut microbiota has a vital role to host animal. However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content. In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform. The valid sequences were classified into 388 Operational Taxonomic Units, and a total of 223 genera, belonging to 20 phyla, were identified. The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions. Proteobacteria and Firmicutes constitute the ‘core’ gut microbiota of blunt snout bream. Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively. Moreover, thirteen potential cellulose-degrading bacteria were detected in our study. The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit. This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit. This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate. Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.

【 授权许可】

CC BY