Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Sociedade Brasileira de Medicina Tropical. Revista

Cabana, Ângela Leitzke¹  Mendes, Josiara Furtado² 

[1] Universidade Federal de Pelotas, Pelotas, Brazil;Universidade Federal do Rio Grande, Rio Grande, Brazil

关键词: Invasive aspergillosis;    Diagnosis;    Platelia® Aspergillus EIA;    Galactomannan;    Air quality.;   

DOI  :  10.1590/0037-8682-0317-2017

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION:Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated.METHODS:We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA).RESULTS:The conidia concentration required for an EIA-positive result was 4.8 × 103 (median).CONCLUSIONS:This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.

【 授权许可】

CC BY