Revista de microbiologia | |
Isolation and characterization of a Pichia anomala strain: a promising candidate for bioethanol production | |
Tao, Nengguo1  | |
[1] Xiangtan University, Xiangtan, China | |
关键词: Bioethanol; gas chromatography analysis; Jiuqu; Pichia anomala.; | |
DOI : 10.1590/S1517-83822011000200031 | |
学科分类:农业科学(综合) | |
来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology | |
【 摘 要 】
A yeast strain designated as Y-1 was isolated and characterized from wine yeast ("Jiuqu"). Based on the morphological and biochemical results, along with the rDNA internal transcribed spacer region (ITS), Y-1 was identified to be a Pichia anomala strain. Y-1 is an ethanol-tolerant strain, enduring ethanol concentrations of up to 14 %. Y-1 growth medium conditions were optimized, results showing good growth in medium with pH ranges from 3.5-6.5, temperature ranges from 25-30 °C, and inoculums range of 8 %-12 %, while optimum growth conditions were reached at a temperature of 30 °C, pH 5.0, and inoculums of 10 %. Furthermore, when the alkaline hydrolyzed Shatian pummelo peel solutions were inoculated with 10 % Y-1 and fermented at 30 °C for 6 d, 4.7 % pure ethanol (w/w) was produced, as evidenced by gas chromatography analysis. Our present study shows potential for the Y-1 strain to be a promising candidate for bioethanol production.
【 授权许可】
CC BY-NC
【 预 览 】
Files | Size | Format | View |
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RO201910258692606ZK.pdf | 1375KB | download |