Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance

Revista de microbiologia

Sharma, S.¹  Bajaj, Bijender K.² 

[1] CCS Haryana Agric. University, Hisar, India;University of Jammu, Jammu, India

关键词: killer yeast;    distillery yeast;    Saccharomyces cerevisiae;    molasses;    ethanol fermentation.;   

DOI  :  10.1590/S1517-83822010000200030

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Saccharomyces cerevisiae HAU-1, a time tested industrial yeast possesses most of the desirable fermentation characteristics like fast growth and fermentation rate, osmotolerance, high ethanol tolerance, ability to ferment molasses, and to ferment at elevated temperatures etc. However, this yeast was found to be sensitive against the killer strains of Saccharomyces cerevisiae. In the present study, killer trait was introduced into Saccharomyces cerevisiae HAU-1 by protoplast fusion with Saccharomyces cerevisiae MTCC 475, a killer strain. The resultant fusants were characterized for desirable fermentation characteristics. All the technologically important characteristics of distillery yeast Saccharomyces cerevisiae HAU-1 were retained in the fusants, and in addition the killer trait was also introduced into them. Further, the killer activity was found to be stably maintained during hostile conditions of ethanol fermentations in dextrose or molasses, and even during biomass recycling.

【 授权许可】

CC BY-NC