Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken

Revista de microbiologia

Barbosa, Fernanda de Oliveira¹  Freitas Neto, Oliveiro Caetano de²  Batista, Diego Felipe Alves³ 

[1] Universidade Estadual Paulista, São Paulo, Brasil;Universidade Federal da Paraíba, Brasil;University of Nottingham, United Kingdom

关键词: Mutation;    Flagellum;    Virulence factors;    Poultry;    Gut colonisation.;   

DOI  :  10.1016/j.bjm.2017.01.012

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ΔmotB and SE ΔfliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE ΔmotB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE ΔfliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken.

【 授权许可】

CC BY-NC