Cytotoxicity and anti-Sporothrix brasiliensis activity of the Origanum majorana Linn. oil

Revista de microbiologia

Madrid, Isabel Martins¹  Ferraz, Vanny²  Picoli, Tony³  Waller, Stefanie Bressan⁴  Cleff, Marlete Brum⁵ 

[1] Prefeitura Municipal de Pelotas, Pelotas, Brazil;Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;Universidade Federal de Pelotas, Brazil;Universidade Federal de Pelotas, Pelotas, Brazil;Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

关键词: Sporotrichosis;    Sporothrix schenckii complex;    Antifungal resistance;    Marjoram;    Lamiaceae.;   

DOI  :  10.1016/j.bjm.2016.07.017

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The study aimed to evaluate the anti-Sporothrix sp. activity of the essential oil of Origanum majorana Linn. (marjoram), its chemical analysis, and its cytotoxic activity. A total of 18 fungal isolates of Sporothrix brasiliensis (n: 17) from humans, dogs and cats, and a standard strain of Sporothrix schenckii (n: 1) were tested using the broth microdilution technique (Clinical and Laboratory Standard Institute - CLSI M27-A3) and the results were expressed in minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). The MIC50 and MIC90 of itraconazole against S. brasiliensis were 2 µg/mL and 8 µg/mL, respectively, and the MFC50 and MFC90 were 2 µg/mL and >16 µg/mL, respectively, with three S. brasiliensis isolates resistant to antifungal. S. schenckii was sensitive at MIC of 1 µg/mL and MFC of 8 µg/mL. For the oil of O. majorana L., all isolates were susceptible to MIC of ≤2.25-9 mg/mL and MFC of ≤2.25-18 mg/mL. The MIC50 and MIC90 were ≤2.25 mg/mL and 4.5 mg/mL, respectively, and the MFC50/90 values were twice more than the MIC. Twenty-two compounds were identified by gas chromatography with a flame ionization detector (CG-FID) and 1,8-cineole and 4-terpineol were the majority. Through the colorimetric (MTT) assay, the toxicity was observed in 70-80% of VERO cells between 0.078 and 5 mg/mL. For the first time, the study demonstrated the satisfactory in vitro anti-Sporothrix sp. activity of marjoram oil and further studies are needed to ensure its safe and effective use.

【 授权许可】

CC BY-NC