Comparison of two agar gel immunodiffusion protocols for diagnosing equine infectious anemia

The Journal of Veterinary Medical Science

Takashi YAMANAKA¹  Manabu NEMOTO²  Hiroshi BANNAI³ 

[1] Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan;Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan;IDEXX Laboratories K.K., 1-22-19 Izumi, Suginami-ku, Tokyo 168-0063, Japan

关键词: agar gel immunodiffusion;    equine infectious anemia;    Japanese act;    OIE;   

DOI  :  10.1292/jvms.18-0103

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

This study compared agar gel immunodiffusion (AGID) protocols for diagnosing equine infectious anemia. Two commercial testing kits were used: one following the Japanese Act on Domestic Animal Infectious Diseases Control and one following the World Organisation for Animal Health (OIE) manual. From 651 samples tested, both protocols gave identical results for 647 samples (23 samples tested positive; 624 tested negative). Non-specific reactions were observed in 21 samples testing negative by the Japanese protocol, but none were observed with the OIE protocol. The kappa coefficient value was 0.962, indicating almost perfect agreement between the two protocols. This study found no difference in diagnostic agreement between the two protocols, but the OIE protocol produced non-specific reactions less frequently than the Japanese protocol.

【 授权许可】

Unknown