Short communication: Local infection of opium poppy leaves by Peronospora somniferi sporangia can give rise to systemic infections and seed infection in resistant cultivars

Spanish Journal of Agricultural Research

Francisco J. Muñoz-Ledesma¹  Miguel Montes-Borrego²  Rafael M. Jiménez-Díaz³ 

[1] Alcaliber S.A., Ctra. Carmona-El Viso del Alcor, km 1.8, Carmona (Sevilla);Instituto de Agricultura Sostenible, CSIC, Apartado 4084, 14080 Córdoba;Instituto de Agricultura Sostenible, CSIC, Apartado 4084, 14080 CórdobaUniversidad de Córdoba, C-4 Celestino Mutis, Campus Rabanales, 14071 Córdoba

关键词: Peronospora meconopsidis;    disease resistance screening;    downy mildew;    inoculum source;    seed transmission;   

DOI  :  10.5424/sjar/2017153-10864

学科分类：农业科学（综合）

来源: I N I A

【 摘 要 】

Downy mildew (DM) of opium poppy ( Papaver somniferum ) caused byPeronospora somniferiis one of the most destructive diseases of this crop due to the systemic nature of infection as compared with local infections caused byPeronospora meconopsidis , the other downy mildew pathogen of this crop. We developed an inoculation method usingPeronospora somniferisporangia as inoculum and demonstrated for the first time that local infection of leaves by sporangia give rise to systemic infections in the plant as well as of seeds. Our results also showed that this inoculation protocol was very effective in reproducing disease symptoms and assessing the resistance response to DM in opium poppy genotypes under field conditions. More interestingly, results indicate that up to 100% of seed samples from some genotypes showing a complete (symptomless) resistant phenotype were infected by the pathogen when seeds were analyzed by aP. somniferi- specific nested-PCR protocol. This latter aspect deserves further attention while breeding opium poppy for resistance toP. somniferi.

【 授权许可】

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