Molecular characterization of a stress-induced NAC gene, GhSNAC3, from Gossypium hirsutum

Journal of genetics

FEI LI^1¹  ZHAN-JI LIU^1² 

[1]College of Life Sciences,Shihezi University, Shihezi 832003, People’s Republic of China^2

[2]Cotton Research Center,Shandong Academy of Agricultural Sciences, Jinan 250100, People’s Republic of China^1

关键词: NAC gene;    GhSNAC3gene;    expression analysis;    drought;    salt;    Gossypium hirsutum;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

NAC genes, specific to plants, play important roles in plant development as well as in response to biotic and abiotic stresses. Here, a novel gene encoding a NAC domain, named as GhSNAC3, was isolated from upland cotton (Gossypium hirsutum L.). Sequence analyses showed that GhSNAC3 encodes a protein of 346 amino acids with an estimated molecular mass of 38.4 kDa and pI of 8.87. Transient localization assays in onion epidermal cells confirmed GhSNAC3 is a nuclear protein. Transactivation studies using a yeast system revealed that GhSNAC3 functions as a transcription activator. Quantitative real-time polymerase chain reaction analysis indicated that GhSNAC3 was induced by high salinity, drought and abscisic acid treatments. We overexpressed GhSNAC3 intobacco by using Agrobacterium-mediated transformation. Transgenic lines produced longer primary roots and more fresh weight under salt and drought stresses as compared to wild-type plants. Collectively, our results indicated that overexpression of GhSNAC3 in tobacco can enhance drought and salt tolerances.

【 授权许可】

CC BY