Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters

BMC Microbiology

Habibollah Esmaily¹  Elham Hajialilo²  Hossein Zarrinfar³  Ali Asghar Najafpoor⁴  Shiva Ghaderifar⁵ 

[1] Allergy Research Center Mashhad, University of Medical Sciences, Mashhad, Iran;Department of Epidemiology and Biostatistics, University of Medical Sciences, Mashhad, Iran;Global Center for Environmental Remediation, University of Newcastle, Callaghan, Iran;Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran

关键词: Ponds water;    Parks;    Acanthamoeba;    Tropical and subtropical region;   

DOI  :  10.1186/s12866-018-1301-x

学科分类：微生物学和免疫学

来源: BioMed Central

【 摘 要 】

Free-living amoeba (FLA) are wide-spread protozoa that are found in different environmental sources including water, soil, dust, hospital units and ventilation areas. These amoebas can act as opportunistic or non-opportunistic pathogens. Among FLAs, some genera such as Acanthamoeba are important because of their potential pathogenic ability in humans. The purpose of this study is to identify of Acanthamoeba isolated from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.From August to December 2015, 90 samples were collected from pond water of parks of 13 regions of Mashhad City. Physicochemical parameters were measured in situ. After filtering, the samples were cultured on Bacto-agar enriched with Escherichia coli. PCR analysis was conducted on the culture-positive samples, and then the PCR products were sequenced. Statistical analysis was performed by SPSS software and Fisher’s Exact and Mann-Whitney test. Among the samples that were examined, 19 samples (21.1%) were positive for Acanthamoeba. The sequencing revealed that Acanthamoeba isolates belonged to T4 genotype of Acanthamoeba. There was no significant relationship between physicochemical parameters and the presence of Acanthamoeba. The prevalence of Acanthamoeba in pond water of parks was relatively high, but there was no significant relationship between physicochemical parameters and the presence of Acanthamoeba.

【 授权许可】

CC BY