Detection of sperm-reactive antibodies in wild sika deer and identification of the sperm antigens

The Journal of Veterinary Medical Science

Osamu KAWASE¹  Mitsuru JIMBO² 

[1] Department of Biology, Premedical Sciences, Dokkyo Medical University, Mibu-machi, Shimotsuga-gun, Tochigi 321-0293, Japan;Department of Marine Biosciences, School of Marine Biosciences, Kitasato University, Kitasato, Sagamihara, Kanagawa 252-0373, Japan

关键词: Cervus nippon;    sika deer;    sperm antigen;    sperm-reactive antibody;   

DOI  :  10.1292/jvms.17-0660

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Antisperm antibodies potentially inhibit sperm functions causing the sterility in humans and experimentally treated animals. However, there is no information about antisperm antibodies emerging spontaneously in wildlife. In this study, we searched for the sperm-reactive antibodies, spontaneously produced in wild sika deer (Cervus nippon), and identified the sperm antigens. We collected 529 fecal masses of sika deer in Japanese cities, from which we extracted the mucosal antibodies to test them for reactivities to deer sperm proteins by ELISA. Two of the extracts contained IgAs that were highly reactive to the sperm proteins. The molecular weights of the active IgAs, partially purified by DEAE-sephadex A-50, were estimated at more than 100 kDa, suggesting that the IgAs evaded drastic digestion in the gastrointestinal tract. Two-dimensional electrophoresis and immunoblotting detected three major antigens, and the following LC-MS/MS analysis identified them as alpha-enolase, phosphoglycerate kinase 2 and acrosin-binding protein. The antibodies were cross-reactive to a recombinant human acrosin-binding protein. To our knowledge, this is the first research to find that the sperm-reactive antibodies are produced spontaneously in wildlife and they recognize a common antigen found in humans.

【 授权许可】

Unknown