NUP58 facilitates metastasis and epithelial-mesenchymal transition of lung adenocarcinoma via the GSK-3β/Snail signaling pathway

American Journal of Translational Research

Wanfang Qiu¹  Chen Li²  Benshan Xiao³  Jianlin Shi⁴  Hui Wang⁵ 

[1]Department of Cell Biology and Medical Genetics, Kunming Medical University, Kunming 650050, China

[2]Department of Gastroenterology, Yanan Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China

[3]Department of Thoracic Surgery, Jiujiang University Clinical Medical College, 57 East Xunyang Road, Jiujiang 332000, China

[4]Department of Thoracic Surgery, Yanan Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China

[5]The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming 650032, China

关键词: NUP58;    EMT;    lung adenocarcinoma;    metastatic mechanism;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

The NUP58 gene encodes a nucleus-pore protein that is a component of nuclear pore complex (NPC). NPC facilitates the transportation of macromolecules (ions and other substances) into the nuclei of eukaryotic cells. However, there are no relevant reports about the NUP58 gene in human lung cancer. In this study, we demonstrated that NUP58 was highly expressed in the primary and metastatic foci of lung adenocarcinoma, with low expression in adjacent tissues and normal lung tissue. In patients with lung adenocarcinoma, the NUP58 gene was highly expressed in patients with stage IV disease (P < 0.05); NUP58 knockdown using a lentiviral vector-mediated shRNA inhibited metastasis and invasion of lung adenocarcinoma cell lines A549 and H1299 in vivo and in vitro. Furthermore, silencing of NUP58 resulted in altered expression of EMT markers, associated GSK-3β/Snail pathways, tumor metastasis and invasion factors. In conclusion, these findings demonstrated that NUP58 can promote the metastasis and invasion of lung adenocarcinoma, which can be partially attributed to the GSK-3β/Snail signaling pathway.

【 授权许可】

CC BY-NC