Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate

Revista de microbiologia

Zhang, Yanqiu¹  Dong, Yuwei² 

[1]China University of Mining and Technology, Xuzhou, China

[2]Xuzhou University of Technology, Xuzhou, China

关键词: Ammonia-oxidizing bacteria;    Immobilization;    Polyvinyl alcohol;    Sodium alginate;    Ammonia oxidation.;   

DOI  :  10.1016/j.bjm.2017.02.001

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Ammonia-oxidizing bacteria were immobilized by polyvinyl alcohol (PVA) and sodium alginate. The immobilization conditions and ammonia oxidation ability of the immobilized bacteria were investigated. The following immobilization conditions were observed to be optimal: PVA, 12%; sodium alginate, 1.1%; calcium chloride, 1.0%; inoculum concentration, 1.3 immobilized balls/mL of immobilized medium; pH, 10; and temperature, 30 °C. The immobilized ammonia-oxidizing bacteria exhibited strong ammonia oxidation ability even after being recycled four times. The ammonia nitrogen removal rate of the immobilized ammonia-oxidizing bacteria reached 90.30% under the optimal immobilization conditions. When compared with ammonia-oxidizing bacteria immobilized by sodium alginate alone, the bacteria immobilized by PVA and sodium alginate were superior with respect to pH resistance, the number of reuses, material cost, heat resistance, and ammonia oxidation ability.

【 授权许可】

CC BY-NC