Effects of human bone morphogenetic protein 2 (hBMP2) on tertiary dentin formation

American Journal of Translational Research

Xia Liu¹  Ying-Jian Sun²  Juan Zhang³  Xiang-Wei Li⁴  Shi-Lei Ni⁵  Xue Zhang⁶ 

[1] Department of Ophthalmology, The Second Hospital of Jilin University, Changchun 130021, Jilin, P. R. China;Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin, P. R. China;Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, Shandong, P. R. China;Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, MD 20892, USA;School and Hospital of Stomatology, Jilin University, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, Jilin, P. R. China;School and Hospital of Stomatology, Jilin University, Key Laboratory of Science and Technology for Stomatology Nanoengineering, Changchun 130021, Jilin, P. R. China

关键词: hBMP2;    formation of tertiary dentin;    dental pulp cells;    gene therapy;    mineralization;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Formation of tertiary dentin to maintain pulp vitality is a major odontoblastic response to dental pulp injury. Human bone morphogenetic protein 2 (hBMP2) can promote proliferation and differentiation of odontoblasts. Current study is interested in evaluating if the hBMP2 can promote the regeneration of tertiary dentin and cure dental pulp injury using the adenoviral vector to deliver hBMP2 cDNA into the pulp. Primary culture of dental pulp cells of exfoliated deciduous teeth (hDPCs) was established. Human serotype 5 adenoviral vector, AdCMV-hBMP2, was created. AdCMV-hBMP2 was used to transduce hDPCs in vitro and dental pulp cells in animal model in vivo. Data clearly demonstrated that hBMP2 increased ALP and mineralization. Reverse transcription-real time quantitative PCR (RT-QPCR) data showed that hBMP2 dramatically increased gene expressions of Runx2 (Runt-related transcription factor 2), ALP, Col Iα (Collagen 1a1), SP7 (Osterix), DMP1 (dentin matrix acidic phosphoprotein 1), DSPP (dentin sialophosphoprotein), and BSP (bonesialoprotein), which are normally involved in osteogenesis/odontogenesis. Data from in vivo assays demonstrated that hBMP2 promoted pulp cell proliferation and increased formation of tertiary dentin in dental pulp. Our in vitro and in vivo data suggest that hBMP2 gene can efficiently be delivered into the dental pulp cells by adenovirus, and show potential clinical application for the treatment of dental pulp damage.

【 授权许可】

CC BY-NC