Constitutive expression of phosphoketolase, a key enzyme for metabolic shift from homo- to heterolactic fermentation in Enterococcus mundtii QU 25

Bioscience of Microbiota, Food and Health

Keisuke NABETA¹  Satoru WATANABE¹  Taku CHIBAZAKURA¹  Mariko SHIMIZU-KADOTA¹  Hirofumi YOSHIKAWA²  Kenji SONOMOTO³  Takeshi ZENDO³ 

[1] Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan;Department of Environmental Systems Sciences, Musashino University, 3-3-3 Ariake, Koto-ku, Tokyo 135-8181, Japan;Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan

关键词: heterolactic fermentation;    metabolic shift;    phosphoketolase activation;    homodimer;    Enterococcus mundtii;    xylose concentration;    constitutive expression;   

DOI  :  10.12938/bmfh.18-030

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

Phosphoketolase (PK) is responsible for heterolactic fermentation; however, the PK gene of Enterococcus mundtii QU 25, xfpA, is transcribed constitutively, even under homolactic fermentation conditions. In order to deduce the regulatory mechanisms of PK activity in QU 25, XfpA levels in QU 25 cells under hetero- and homolactic fermentation conditions were tested using western blotting. The results showed that the XfpA protein expression was similar under both conditions and that the expression products formed complexes, most likely homodimers, indicating that the regulation of PK activity is downstream of translation.

【 授权许可】

CC BY