Nested Polymerase Chain Reaction with Specific Primers for Mucorales in the Serum of Patients with Hematological Malignancies

Japanese journal of infectious diseases

Mitsuhito Hirano¹  Yasunori Ota²  Tomohiko Koibuchi³  Tomomi Takei⁴ 

[1] Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo;Department of Pathology, Research Hospital, Institute of Medical Science, University of Tokyo;Infectious Diseases and Applied Immunology, Research Hospital, Institute of Medical Science, University of Tokyo;Laboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo

关键词: nested polymerase chain reaction (nested PCR);    Mucorales;    mucormycosis;    opportunistic infection;    serum;   

DOI  :  10.7883/yoken.JJID.2018.379

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Mucormycosis is an opportunistic infection occurring in immunocompromised hosts with hematological malignancies. Mortality due to mucormycosis in patients with hematological malignancies is high. However, the clinical symptoms of mucormycosis are poorly characterized, and diagnosis is difficult due to the lack of specific culture or serological markers or antigens. We present two cases in which nested polymerase chain reaction with specific primers was used in the serum of patients with hematological malignancies.

【 授权许可】

Unknown