Changes in gut microbiota and plasma inflammatory factors across the stages of colorectal tumorigenesis: a case-control study

BMC Microbiology

Yongzhen Zhang¹  Na Wang²  Enda Yu³  Quancai Cai⁴  Xin Yu⁵ 

[1] Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai, China;Department of General Surgery, Changhai Hospital, Naval Medical University, Shanghai, China;Key Laboratory of Public Health Safety of Ministry of Education, School of Public Health, Fudan University, Shanghai, China;School of Epidemiology and Public Health, University of Ottawa Faculty of Medicine, Ottawa, Canada;The 92914th Military Hospital of PLA, Shanghai, China

关键词: Colorectal cancer (CRC);    Gut microbiota;    Plasma inflammatory factors;    Correlation analysis;   

DOI  :  10.1186/s12866-018-1232-6

学科分类：微生物学和免疫学

来源: BioMed Central

【 摘 要 】

Colorectal cancer (CRC) is a common malignant gastrointestinal tumor. In China, CRC is the 5th most commonly diagnosed cancer. The vast majority of CRC cases are sporadic and evolve with the adenoma-carcinoma sequence. There is mounting evidence indicating that gut microbiota and inflammation play important roles in the development of CRC although study results are not entirely consistent. In the current study, we investigated the changes in the CRC-associated bacteria and plasma inflammatory factors and their relationships based on data from a case-control study of Han Chinese. We included 130 initially diagnosed CRC patients, 88 advanced colorectal adenoma patients (A-CRA), 62 patients with benign intestinal polyps and 130 controls. Fecal microbiota composition was obtained using 16S ribosomal DNA (16S rDNA) sequencing. PCOA analysis showed structural differences in microbiota among the four study groups (P = 0.001, Unweighted Unifrac). Twenty-four CRC-associated bacteria were selected by a two-step statistical method and significant correlations were observed within these microbes. CRC-associated bacteria were found to change with the degree of malignancy. Plasma C-reactive protein (CRP) and soluble tumor necrosis factor II (sTNFR-II) displayed significant differences among the four study groups and increased with adenoma-carcinoma sequence. The correlations of CRP and sTNFR-II with several CRC-associated microbes were also explored. CRC-associated species and plasma inflammatory factors tended to change along the adenoma-carcinoma sequence. Several CRC-associated bacteria were correlated with CRP and sTNFR-II. It is likely that gut microbiome and inflammation gradually form a microenvironment that is associated with CRC development.

【 授权许可】

CC BY