Growth rate inhibition of phytopathogenic fungi by characterized chitosans

Revista de microbiologia

Oliveira Junior, Enio N.¹  Gueddari, Nour E. El² 

[1] Universidade Estadual de Campinas;University of Münster, Münster, Germany

关键词: chitosan;    antifungal activity;    fungal morphology;    phytopathogenic fungi.;   

DOI  :  10.1590/S1517-83822012000200046

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The inhibitory effects of fifteen chitosans with different degrees of polymerization (DP) and different degrees of acetylation (FA) on the growth rates (GR) of four phytopathogenic fungi (Alternaria alternata, Botrytis cinerea, Penicillium expansum, and Rhizopus stolonifer) were examined using a 96-well microtiter plate and a microplate reader. The minimum inhibitory concentrations (MICs) of the chitosans ranged from 100 µg × mL-1 to 1,000 µg × mL-1 depending on the fungus tested and the DP and FA of the chitosan. The antifungal activity of the chitosans increased with decreasing FA. Chitosans with low FA and high DP showed the highest inhibitory activity against all four fungi. P. expansum and B. cinerea were relatively less susceptible while A. alternata and R. stolonifer were relatively more sensitive to the chitosan polymers. Scanning electron microscopy of fungi grown on culture media amended with chitosan revealed morphological changes.

【 授权许可】

CC BY-NC