GATA4 negatively regulates bone sialoprotein expression in osteoblasts

BMB Reports

Yong Jun Choi^4¹  Yoon-Sok Chung^4²  Byung-chul Jeong^2,3³  Insun Song^1⁴ 

[1]Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469^3

[2]Department of Endocrinology, Ajou University, Suwon 16499, Korea^4

[3]Pharmacology and^2

[4]Shool of Biological Sciences, Seoul National University, Seoul 08826, Departments of^1

关键词: Bone sialoprotein;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

GATA4 has been reported to act as a negative regulator in osteoblast differentiation by inhibiting the Dlx5 transactivation of Runx2 via the attenuation of the binding ability of Dlx5 to the Runx2 promoter region. Here, we determine the role of GATA4 in the regulation of bone sialoprotein (Bsp) in osteoblasts. We observed that the overexpression of Runx2 or Sox9 induced the Bsp expression in osteoblastic cells. Silencing GATA4 further enhanced the Runx2- and Sox9- mediated Bsp promoter activity, whereas GATA4 overexpression down-regulated Bsp promoter activity mediated by Runx2 and Sox9. GATA4 also interacted with Runx2 and Sox9, by attenuating the binding ability of Runx2 and Sox9 to the Bsp promoter region. Our data suggest that GATA4 acts as a negative regulator of Bsp expression in osteoblasts.

【 授权许可】

Unknown