The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China

Revista de microbiologia

Zhang, Jiu-ming²  Yao, Xie-feng³  Tian, Li⁴  Guo, Jian-hua⁵ 

[1] Technology, Qingdao, China;First Institute of Oceanography, Qingdao, China;Institute of Vegetable Crops, Nanjing, China;Qingdao University of Science &amp

关键词: Heavy metal contamination;    PCR-DGGE fingerprinting;    Multivariate analysis;    Bacterial community structure.;   

DOI  :  10.1016/j.bjm.2016.09.007

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure.

【 授权许可】

CC BY-NC