Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films

Proceedings

Becer, C. Remzi¹  Colak, Burcu²  Gibbs, Martin John³  Watkinson, Michael⁴  Ahmad, Norlaily⁵  Zhang, De-Wen⁶ 

[1] Author to whom correspondence should be addressed.;Centre of Foundation Studies, Universiti Teknologi MARA, Cawangan Selangor, Kampus Dengkil, Dengkil 43800, Malaysia;Institute of Medical Engineering, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China;Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018;School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, UK;The Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Staffordshire ST5 5BG, UK

关键词: collagenase biosensor;    peptide cross-linked;    hydrogel;    quartz crystal microbalance;    degradation;    poly(ethylene glycol);   

DOI  :  10.3390/proceedings2130961

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Peptide cross-linked poly(ethylene glycol) hydrogel is a promising biomaterial that has been used widely for drug delivery and tissue engineering. However, the use of this material as a biosensor material for the detection of collagenase has not been explored. Collagenase play a key role in rheumatoid arthritis and osteoarthritis. Detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals and their degradation in the presence of collagenase was monitored using a Quartz Crystal Microbalance (QCM). The biosensor was shown to respond to concentrations between 2 nM to 2000 nM with a lower detection limit of 2 nM.

【 授权许可】

CC BY