Baicalein suppress EMT of breast cancer by mediating tumor-associated macrophages polarization

American Journal of Cancer Research

Jizhao Wang¹  Xixi Zhao²  Xu Liu³  Jingkun Qu⁴ 

[1] Department of Blood, The Chengdu Military Command General Hospital of Kunming Medical University, 212 West Daguan Road, Kunming 650032, Yunnan, P. R. China;Department of Oncology, The Second Affiliated Hospital of Xian Jiaotong University, 157 West Fifth Street, Xian 710004, Shaanxi, P. R. China;Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, 300 Xueshi Street, Changsha 410208, Hunan, P. R. China;The Second Department of Thoracic Surgery, The First Affiliated Hospital of Xian Jiaotong University, 277 West Yanta Road, Xian 710061, Shaanxi, P. R. China

关键词: Baicalein;    TAMs;    TGF-β;    1;    EMT;    breast cancer;   

DOI  :  

学科分类：肿瘤学

来源: e-Century Publishing Corporation

【 摘 要 】

Tumor associated macrophages (TAMs) are the main infiltrating component in the tumor microenvironment and play an important role in cancer progression. Baicalein has a wide range of pharmacological properties. This study explores the potential effect of baicalein on macrophages polarization and epithelial-mesenchymal transition (EMT) of breast cancer. Co-culture system was established to evaluate the interaction between TAMs and breast cancer cells. Then the role of baicalein in modulating TAMs function was assessed. Finally, breast cancer mouse model was established to study the underlying mechanism. In vitro experiments showed that co-culture with M2 macrophages significantly enhanced EMT of both MDA-MB-231 and MCF-7 breast cancer cells. Baicalein could regulate polarization of M2 and attenuate TGF-β1 secretion. In vivo experiments showed that compared with the MDA-MB-231 + M2 group, tumor growth and metastasis of baicalein + MDA-MB-231 + M2 group was significantly inhibited, with smaller tumor size and decreased lung metastasis lesions. Our findings suggest that the regulation of TAMs may be a novel mechanism underlying the anti-tumor effects of baicalein in breast cancer.

【 授权许可】

CC BY-NC