The precursor osteoblast-like cell, MC3T3-E1 cell line, enhances sodium–calcium exchanger 1 (Ncx1) gene expression by stretch stimuli prior to osteoblast differentiation

The Journal of Veterinary Medical Science

Ryo AOKI¹  Katsuhiko WARITA²  Naoki KITAMURA³ 

[1] Laboratory of Basic Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan;Veterinary Anatomy, Department of Veterinary Science, Faculty of Agriculture, Tottori University, 4-101 Koyama Minami, Tottori 680-8553, Japan;Veterinary Physiology, Department of Veterinary Science, Faculty of Agriculture, Tottori University, 4-101 Koyama Minami, Tottori 680-8553, Japan

关键词: Ncx1;    Ncx3;    osteoblast differentiation;    stretch stimulus;    Trpv4;   

DOI  :  10.1292/jvms.18-0766

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

This study evaluated the expression of genes involved in the concentration of Ca2+ in precursor osteoblast-like cell, MC3T3-E1 subjected to stretching stimuli. Transient receptor potential vanilloid 4 (Trpv4) gene expression, the factor that is activated by stretch stimulation and enables inflow of Ca2+ from the extracellular space, was not affected as a result of stretch stimulation; conversely, the expression of sodium-calcium exchanger 1 (Ncx1) gene involved in outflow of intracellular Ca2+ increased, depending on stimulation intensity. Localization of Ca2+ correlated with the positioning of the endoplasmic reticulum, and intracellular Ca2+ decreased in inverse proportion to the intensity of the stretching force. These results suggest that stretch stimulation activates intracellular Ca2+ elimination rather than Ca2+ uptake before osteoblast differentiation.

【 授权许可】

Unknown