Anti-CD200 attenuates concanavalin A induced hepatitis via modulating the imbalance of CD4+ T lymphocyte differentiation in mice

American Journal of Translational Research

Zhong Man¹  Chuan Geng²  Xiaohong Zhao³  Shuo Ding⁴ 

[1] Department of Anesthesiology, Affiliated Hospital of Guilin Medical University, 15 Lequn Rd, Guilin 541001, China;Department of Anesthesiology, Fengxian Peoples Hospital, 51 West Renmin Rd, Fenxian, Xuzhou 221700, China;Department of Anesthesiology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gongwei Road, Huinan Town, Pudong, Shanghai 201399, China;Department of Intensive Care, Fengxian Peoples Hospital, 51 West Renmin Rd, Fenxian, Xuzhou 221700, China

关键词: CD200;    Hepatitis;    CD4+ T lymphocyte differentiation;    mice;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Hepatitis occurs in critical ill patients with bad morbidity and mortality. It is known that imbalance of Th1 and Th2 lymphocytes differentiations plays a key role in its mechanisms. Recent studies indicated that type 1 membrane glycoprotein CD200 serves as co-inhibitory molecule, negatively regulating the immune response. In regard of this, we used Concanavalin A (Con A) induced liver injury model to research the effect of CD200 on the differentiation of CD4+ T lymphocyte and found that the expression of CD200 on CD4+ T was significantly higher in hepatitis mouse. The apoptosis of CD4+ T cell in Con A induced liver injury was significantly attenuated by anti-CD200. The concentration of solube IL-2 and IFN-γ was reduced by anti-CD200, in addition, the expression of T-bet, GATA3 and FoxP3 mRNA were all attenuated by anti-CD200. The phosphorylation of SH-2 containing inositol 5’ polyphosphatase 1 (SHIP1) was significantly increased in Con A induced liver injury and reduced by anti-CD200. We hypothesized that, anti-CD200 inhibited the phosphorylation of SHIP1, the expression of T-bet, GATA3 and FoxP3 mRNA and CD4+ T differentiation to protect the liver from autoimmune hepatitis.

【 授权许可】

CC BY-NC