Oxidative stress and antioxidant response in a thermotolerant yeast

Revista de microbiologia

Aguilera-Aguirre, Leopoldo¹  Mejía-Barajas, Jorge A.²  Salgado-Garciglia, Rafael³  Montoya-Pérez, Rocío⁴ 

[1]Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Guadalajara, Mexico

[2]Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico

[3]Universidade Nacional Autónoma de Mexico, Tlalnepantla, Mexico

[4]University of Texas Medical Branch at Galveston, Galveston, USA

关键词: Yeast;    Increased temperature;    Oxygen-derived free radicals;    Thermotolerant.;   

DOI  :  10.1016/j.bjm.2016.11.005

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Stress tolerance is a key attribute that must be considered when using yeast cells for industrial applications. High temperature is one factor that can cause stress in yeast. High environmental temperature in particular may exert a natural selection pressure to evolve yeasts into thermotolerant strains. In the present study, three yeasts (Saccharomyces cerevisiae, MC4, and Kluyveromyces marxianus, OFF1 and SLP1) isolated from hot environments were exposed to increased temperatures and were then compared with a laboratory yeast strain. Their resistance to high temperature, oxidative stress, and antioxidant response were evaluated, along with the fatty acid composition of their cell membranes. The SLP1 strain showed a higher specific growth rate, biomass yield, and biomass volumetric productivity while also showing lower duplication time, reactive oxygen species (ROS) production, and lipid peroxidation. In addition, the SLP1 strain demonstrated more catalase activity after temperature was increased, and this strain also showed membranes enriched in saturated fatty acids. It is concluded that the SLP1 yeast strain is a thermotolerant yeast with less oxidative stress and a greater antioxidant response. Therefore, this strain could be used for fermentation at high temperatures.

【 授权许可】

CC BY-NC