Synthesis and characterization of porous cytocompatible scaffolds from polyvinyl alcohol–chitosan

Bulletin of Materials Science

MAHDI ZOLGHADRI¹  SAEED SABER-SAMANDARI^1² 

[1] New Technologies Research Center, Amirkabir University of Technology, Tehran 1591634653, Iran^1;Sustainable and Renewable Energy Engineering (SREE), University of Sharjah, Sharjah 2727227272, UAE^2

关键词: Bone tissue engineering;    nanoclay particles;    polyvinyl alcohol;    chitosan.;   

DOI  :  

学科分类：材料工程

来源: Indian Academy of Sciences

【 摘 要 】

In this study, novel porous cytocompatible scaffolds with a 3D nanocomposite structure were synthesized by using nanoclay particles embedded into a biopolymer blend composed of polyvinyl alcohol (PVA) and chitosan (CS).According to the results, the Fourier transform infrared spectrum confirmed the presence of nanoclay, PVA and CS in the scaffold structure. X-ray diffraction outcomes showed the enhancement of crystalline zone in the synthesized 3D scaffolds by increasing the nanoclay content. Scanning electron microscopy (SEM) images revealed the highly porous interconnected microstructure of the scaffolds. Also, the energy-dispersive X-ray spectra verified the presence of nanoclay, PVA and CS in the sample with the highest nanoclay content. According to mechanical properties and porosity of the synthesized 3Dscaffolds, compressive strength (i.e., $3.5 \pm 0.2$ MPa), elastic modulus ($1.42 \pm 0.02$ GPa) and porosity (75–82%) of the sample with the highest nanoclay content was in the range of mechanical properties and porosity of a natural trabecular bone tissue. The swelling of samples in a phosphate-buffered saline solution was less than the swelling in water. In addition, increasing the content of nanoclay decreases the percentage of swelling. Outcomes of cell culture experiments confirmed that the synthesized 3D scaffolds were not toxic and the cell attachment SEM images showed a sufficient attachment of the cell to the interconnected porous structure of the sample. Results suggest that the synthesized 3D scaffold in this study possesses proper microstructure properties and no cytotoxicity to be replaced with natural bone tissues.

【 授权许可】

CC BY