Isolation and purification of complex II from proteus mirabilis strain ATCC 29245

Revista de microbiologia

Shabbiri, Khadija¹  Ahmad, Waqar² 

[1] IGC University, Lahore, Pakistan;Pakistan Council of Scientific and Industrial Research Labs Complex, Lahore

关键词: Proteus mirabilis;    complex II;    cytochrome b.;   

DOI  :  10.1590/S1517-83822010005000004

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism.

【 授权许可】

CC BY-NC