Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)

The Journal of Veterinary Medical Science

Manabu NEMOTO¹  Kazuya TAKEHANA²  Keita MATSUNO³  Teruo KINJYO⁴ 

[1] Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan;Ichihara Elephant Kingdom Zoological Park, Ichihara, Chiba 290-0521, Japan;Laboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan;Okinawa Zoo and Museum, Okinawa-shi, Okinawa 904-0021, Japan

关键词: acute hemorrhagic disease;    Asian elephants (Elephas maximus);    elephant endotheliotropic herpesvirus (EEHV);    loop-mediated isothermal amplification (LAMP);    polymerase chain reaction (PCR);   

DOI  :  10.1292/jvms.18-0683

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 101.2 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease.

【 授权许可】

Unknown