Identification of Three Distinct Groups of Anopheles lindesayi in Japan by Morphological and Genetic Analyses

Japanese journal of infectious diseases

Toshihiko Sunahara¹  Hwa-Jen Teng²  Yukiko Higa³  Nozomi Imanishi⁴ 

[1]Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare

[2]Department of Medical Entomology, National Institute of Infectious Diseases

[3]Department of Vector Ecology and Environment, Institute of Tropical Medicine (NEKKEN), Nagasaki University

[4]Leading Program, Graduated School of Biomedical Sciences

关键词: Anopheles lindesayi;    malaria vector;    COI;    ITS2;    Japan;   

DOI  :  10.7883/yoken.JJID.2017.537

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Anopheles (Anopheles) lindesayi Giles consists of 5 subspecies. In Japan, only one subspecies, An. l. japonicus Yamada, has been reported. Its geographical populations are morphologically diverse; however, they are regarded as a single subspecies. In this study, we re-evaluated the taxonomic status of An. l. japonicus in Japan, and that of another subspecies, An. l. pleccau, distributed in Taiwan, by comparative morphological and molecular analyses based on the gene sequences of mitochondrial DNA cytochrome c oxidase I (COI) and ribosomal DNA internal transcribed spacer 2 (ITS2). Nucleotide sequence divergence was calculated using the Kimura-two-parameter (K2P) distance model. Phylogenetic trees based on COI and ITS2 sequences showed 3 distinct clades: Eastern Japan, Western Japan, and the Ryukyus. The sequences of the Ryukyu specimens were located within the same clade as that of the sequences of the Taiwanese specimens. Regarding the COI sequences, the 3 geographical groups in Japan were genetically distinct. The following morphological characteristics distinguished the groups: larval seta 1-S, pupal setae 5 through segments IV–VII, and pupal setae 6 on segments IV–VII. Based on these results, it was revealed that An. l. japonicus included 3 genetically and morphologically distinct groups: 2 groups of An. l. japonicus and a group in the Ryukyus, which was a synonym of An. l. pleccau.

【 授权许可】

Unknown