Quantification of Folic Acid in Human Serum Using Isotope Dilution Ultra‐High‐Pressure Liquid Chromatography/Mass Spectrometry

Bulletin of the Korean Chemical Society

SeokWon Hyung¹  Joonhee Lee² 

[1] Division of Chemical and Medical Metrology Korea Research Institute of Standards and Science Daejeon 34113 South Korea;Hygienic Safety and Analysis Center World Institute of Kimchi Gwangju 61755 South Korea

关键词: Folic acid;    Human serum;    Ultra‐;    high‐;    pressure liquid chromatography;    Isotope dilution mass spectrometry;   

DOI  :  10.1002/bkcs.11356

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Analytical method for an accurate determination of folic acid in human serum has been established using isotope dilution ultra‐high‐pressure liquid chromatography/mass spectrometry (ID‐UPLC/MS). Sample extract was prepared from 0.5 mL of human serum spiked with 13C5‐folic acid solution and then LC–MS/MS experiment was performed on multiple reaction monitoring mode for 20 min. The method was tested for repeatability using homogeneous human serum and it showed good repeatability with relative standard deviation of 1.8%. Further evaluation was carried out through NIST SRM 1955 samples (level I, II, and III) and the measurement results were in good agreement with the certified values with good reproducibility over two periods. It was also revealed that the obtained relative expanded uncertainty was 12% which is much smaller than that of previously reported methods, indicating that this method is very reliable and accurate for determining the content of folic acid in human serum.

【 授权许可】

Unknown