Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68

Revista de microbiologia

Hamidi-Esfahani, Zohreh¹  Samadlouie, Hamid-Reza²  Alavi, Seyed-Mehdi³ 

[1] National institute for Genetic Engineering and Biotechnology, Tehran, Iran;Tarbiat Modares University, Tehran, Iran;Universiti Sains Malaysia, Pinang, Malaysia

关键词: Mortierella alpina;    arachidonic acid;    lipid;    gene expression;    real time PCR.;   

DOI  :  10.1590/S1517-83822014000200010

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.

【 授权许可】

CC BY-NC