In vitro antiproliferative studies of extracts of the marine molluscs: Tympanatonus fuscatus Var radula (linnaeus) and Pachymelania aurita (muller)

International Journal of Biochemistry and Molecular Biology

Naomi Maina¹  Omolaja Osoniyi²  Queensley Eghianruwa³  Sabina Wachira⁴ 

[1]Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya

[2]Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile Ife, Nigeria

[3]Department of Molecular Biology and Biotechnology, Pan African University Institute of Science, Technology and Innovation, Kenya

[4]Kenya Medical Research Institute, Nairobi, Kenya

关键词: Tympanatonus fuscatus;    Pachymelania aurita;    allium cepa;    Hep2;    DU145;    HCC1395;    Vero E6;    antimitotic;    antiproliferative;    CASP3;    P53;   

DOI  :  

学科分类：生物化学/生物物理

来源: e-Century Publishing Corporation

【 摘 要 】

This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone-methanol and aqueous extracts of two marine molluscs commonly found in the Niger Delta region of Nigeria; T.fuscatus and P.aurita, against human cancerous cell lines (DU145, Hep-2, and HCC1395) cell lines in vitro. The antimitotic activity of the extracts was evaluated using Allium cepa root meristematic cells. Antiproliferative activity of the plant extracts against the cancerous cell lines was compared with normal cell line (VeroE6). Doxorubicin was used as a positive control. Gene expression studies using qPCR for the proapoptotic genes, CASP3, CASP8 and P53 were also carried out. The alcohol extract of T.fuscatus (TFAC) exhibited the most promising activity against all the cancer cell lines tested (DU145 IC50 = 96.48 ± 1.36 μg/ml, HCC 1395 IC50 = 61.44 ± 2.45 μg/ml, Hep2 IC50 = 0.52 ± 0.36 μg/ml) and also had the highest selectivity index of 4.94, 7.78 and 921.97 for DU145, HCC 1395 and Hep-2 cells respectively. Furthermore, TFAC was the only extract that significantly upregulated the expression of caspase 3, caspase 8 and P53. Thus, these findings suggest potential exploitation of TFAC as an anticancer agent.

【 授权许可】

CC BY-NC