Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

BMB Reports

Sanghoo Lee^1¹  Hwan-Sub Lim^2²  Hye-Jin Shin^1³  Soonho Kwon^1⁴ 

[1]Department of Bioanalytical and Mass Spectrometry, Seoul Medical Science Institute &^1

[2]Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA^4

[3]Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang 412-270, Korea^3

[4]Seoul Clinical Laboratories, Seoul 152-766, Korea^2

关键词: Diurnal rhythms;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

The levels of salivary cortisol and cortisone in Korean adults were measured for the first time using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The salivary cortisol and cortisone were separated within 10 min. The regression coefficients (r) of the calibration curves were greater than 0.999 for the two steroids. The limits of quantitation (LOQ) were 0.2 ng/ml for cortisol and 1 ng/ml for cortisone. The intra-day precisions of the assay were <3.9% and 8.6% for cortisol and cortisone respectively, and the inter-day precisions were <1.9% and 4.3% for cortisol and cortisone, respectively. The salivary cortisone concentrations were approximately 4-9 times higher than those of salivary cortisol during the daytime. Diurnal rhythms, during which the cortisol and cortisone concentrations were higher in the morning than in the afternoon, were also observed. The present assay may be useful for the diagnosis of several adrenal dysfunctions in clinical biochemistry.

【 授权许可】

Unknown