STRAIGHTFORWARD, ECONOMICAL PROCEDURES FOR MICROSCALE ELLMAN'S TEST FOR CHOLINESTERASE INHIBITION AND REACTIVATION

Quimica nova

Cavalcante, Samir F. de A.¹  Kitagawa, Daniel A. S.² 

[1] Exército Brasileiro, Rio de Janeiro, Brazil;Universidade Federal do Rio de Janeiro, Brazil

关键词: Ellman's test;    cholinesterase;    neurodegenerative diseases;    nerve agents;    pesticides.;   

DOI  :  10.21577/0100-4042.20170278

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Straightforward, microscale, Ellman's cholinesterase test procedures are presented, useful for screening of novel compounds aiming at neurodegenerative diseases and antidotes towards nerve agents and pesticides. Herein, we detail the different procedure features and set forth guidelines on experimental choices researchers may make. The detailed procedure lowers the costs for Ellman´s test, what is highly desirable for low-budget groups in science-lagging and developing countries.

【 授权许可】

CC BY