Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

BMB Reports

Ok Tae Kim^3¹  Sang Hee Shim^1²  Se-Weon Lee^4³  Sung Hong Kim^2⁴  Mijeong Cha^1⁵ 

[1] Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707^4;Analysis Research Division, Daegu Center, Korea Basic Science Institute, Daegu 702-701^2;Green Bio Research Center, KRIBB, Daejeon 305-806, Korea^5;Planning and Coordination Division, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706^3;School of Biotechnology, Yeungnam University, Gyeongsan 712-749^1

关键词: Panax ginseng C.A. Meyer;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated 9.1 μg taxadiene per gram of dry weight. In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and 15.9 μg per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene.

【 授权许可】

Unknown