Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Plasma

Talmon, Maria¹  Gherardi, Matteo²  Colombo, Vittorio³  Crestale, Letizia⁴  Laurita, Romolo⁵  Bisag, Alina⁶  Liguori, Anna⁷  Stancampiano, Augusto⁸  Amoruso, Angela⁹ 

[1]D Biolab, 28100 Novara, Italy

[2]Authors to whom correspondence should be addressed.

[3]Current address: GREMI, UMR7344 CNRS/Université d’Orléans, 45067, Orléans, France

[4]Department of Health Sciences, University of Piemonte Orientale, 28100 Novara, Italy

[5]Department of Industrial Engineering, Alma Mater Studiorum-Università di Bologna, 40100 Bologna, Italy

[6]Interdepartmental Centre for Industrial Research Agrifood, Alma Mater Studiorum-Università di Bologna, 47521 Cesena, Italy

[7]Interdepartmental Centre for Industrial Research, Advanced Applications in Mechanical Engineering and Materials Technology, Alma Mater Studiorum-Università di Bologna, 40100 Bologna, Italy

[8]R &

[9]The authors have contributed equally.

关键词: cold atmospheric pressure plasma;    dielectric barrier discharges;    monocytes;    monocytes-derived macrophages;   

DOI  :  10.3390/plasma1020023

学科分类：原子、分子光学和等离子物理

来源: mdpi

【 摘 要 】

Monocytes are involved in innate immune surveillance, establishment and resolution on inflammation, and can polarize versus M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. The possibility to control and drive immune cells activity through plasma stimulation is therefore attractive. We focused on the effects induced by cold-atmospheric plasma on human primary monocytes and monocyte-derived macrophages. Monocytes resulted more susceptible than monocyte-derived macrophages to the plasma treatment as demonstrated by the increase in reactive oxygen (ROS) production and reduction of viability. Macrophages instead were not induced to produce ROS and presented a stable viability. Analysis of macrophage markers demonstrated a time-dependent decrease of the M1 population and a correspondent increase of M2 monocyte-derived macrophages (MDM). These findings suggest that plasma treatment may drive macrophage polarization towards an anti-inflammatory phenotype.

【 授权许可】

CC BY