Histopathological evaluation of mesenchymal stem cells in the healing of anastomosed carotid arteries

American Journal of Stem Cells

Keramettin Aydın¹  Ercan Yarar²  Cengiz Çokluk³  Enis Kuruoğlu⁴  Eyüp Genç⁵  Adnan Altun⁶ 

[1] Department of Medical Biology, Ondokuz Mayis University, Samsun, Turkey;Department of Neurosurgery, Bafra State Hospital, Sinop, Turkey;Department of Neurosurgery, Ondokuz Mayis University, Samsun, Turkey;Department of Neurosurgery, Turhal State Hospital, Tokat, Turkey;Department of Pathology, Ondokuz Mayis University, Samsun, Turkey;Neurosurgery, Medicana Private Hospital, Samsun, Turkey

关键词: End to end carotid arteries anastomosis;    local application of mesenchyme stem cells;    histopathological evaluation;    thrombosis;   

DOI  :  

学科分类：分子生物学，细胞生物学和基因

来源: e-Century Publishing Corporation

【 摘 要 】

The objective of this study was to evaluate the influence of mesenchymal stem cells on the healing of experimental carotid artery anastomoses histopathologically. Twenty-four female Sprague-Dawley rats were used in this study. After random separation of the subjects into two groups, in both groups carotid arteries were transected and anastomosed in end-to-end fashion. Anastomoses were locally treated with 1 ml 0.09% NaCl, and 1 ml mesenchymal stem cell suspension (1×106 cells) in control and trial groups, respectively. Anastomoses were wrapped with an 8 mm sheet of surgicel and soaked with BioGlue in order to sequestrate the stem cells. After patencies were confirmed via Doppler USG, surgical site was closed with 2/0 silk sutures. Histopathological evaluation was carried out after 4 weeks. In respect to endothelial continuity, vessel patency (along with presence or absence of restenosis), integrities of internal and external elastic laminae, muscularis and adventitia; no statistically significant differences were present between the trial and control groups. In Trial and Control Groups, luminal thrombus was present in 8 (66.6%) and 3 (25%) of the 12 subjects, respectively. The difference was statistically significant (P < 0.05). Recanalization was present in 6 subjects in trial group; 1 subjects in Control Group, respectively. Our results suggest that local administration of mesenchyme stem cell does not have a positive influence on success of an anastomosis.

【 授权许可】

CC BY-NC