期刊论文详细信息
Revista de microbiologia
Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi
Primaz, Andreiza Lazzarotto1  Colla, Luciane Maria2 
[1] Federal University Foundation of Rio Grande, Rio Grande, Brazil;University of Passo Fundo, Passo Fundo, Brazil
关键词: Agricultural residues;    Lipase;    Optimization;    Submerged bioprocess.;   
DOI  :  10.1016/j.bjm.2016.01.028
学科分类:农业科学(综合)
来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology
PDF
【 摘 要 】

A Plackett–Burman Factorial Design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. The factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (Aspergillus niger or Aspergillus flavus were selected as good lipase producers via submerged fermentation), pH and agitation. The concentration of the yeast extract and soybean oil and the pH had a significant effect (p < 0.05) on lipase production and were consecutively studied through a Full Factorial Design 23, with the concentration of yeast extract and pH being significant (p < 0.05). These variables were optimized using a central composite design, obtaining maximum lipolytic activities with the use of 45 g/L of yeast extract and pH 7.15. The statistical model showed a 94.12% correlation with the experimental data.

【 授权许可】

CC BY-NC   

【 预 览 】
附件列表
Files Size Format View
RO201910255194770ZK.pdf 1265KB PDF download
  文献评价指标  
  下载次数:15次 浏览次数:5次