Short communication: Identification of cultivated and wild Vaccinium species grown in Portugal

Spanish Journal of Agricultural Research

Valdemar Carnide¹  Márcia Carvalho²  Manuela Matos⁴ 

[1] Integrative Sciences Institute, Campo Grande, Lisboa;University of Trás-os-Montes and Alto Douro (UTAD), Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), 5000-801 Vila Real;University of Trás-os-Montes and Alto Douro (UTAD), Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), 5000-801 Vila RealUniversity of Lisboa, Faculty of Sciences, BioISI– Biosystems &University of Trás-os-Montes and Alto Douro (UTAD), Dept. of Genetics and Biotechnology, 5000-801 Vila RealUniversity of Lisboa, Faculty of Sciences, BioISI– Biosystems &

关键词: blueberry;    bilberry;    genetic diversity;    differentiation;    molecular marker;   

DOI  :  10.5424/sjar/2018163-12502

学科分类：农业科学（综合）

来源: I N I A

【 摘 要 】

Vacciniumcrops offer a variety of benefits for human health due their high levels of antioxidants. Genetic diversity between twoVacciniumspecies (sixteen cultivars ofVaccinium corymbosumand three wild populations ofVaccinium myrtillus ) were evaluated using Inter Simple Sequence Repeat (ISSR) markers. InV. corymbosum74 polymorphic markers corresponding to 83.2% of polymorphism were obtained while inV. myrtillusonly four polymorphic markers corresponding to 83.2% and 10.6% of polymorphism were observed. The dendrogram obtained showed a clear division into two distinct groups corresponding to the two analyzed species.V. corymbosumgroup is divided in different sub-clusters based on cultivars pedigree relationships. Twenty-eight specific bands were detected in total; 6 forV. corymbosumand 22 forV. myrtillus . Results allowed the selection of five primers due it potential to detect specific bands in the two species. These markers could be useful for identifying species and cultivars and consequently help in the management of germplasm collections and in breeding programs.

【 授权许可】

Free