In Vitro Antioxidant Activity and Total Phenolic Content of Root Extracts from Phragmanthera glaucocarpa (Peyr.) Balle

Jundishapur Journal of Natural Pharmaceutical Products

Nelson António Freitas Fernandes¹  Dina Isabel Malheiros Dinis de Mendonça²  Laura Isabel Nave Canelo³  Rosalina da Conceição dos Santos Mata⁴ 

[1] CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal;Chemistry Department, Agostinho Neto University, Luanda, Angola;Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, Ohio, United States;Materiais Fibrosos e Tecnologias Ambientais-FibEnTech, University of Beira Interior, Covilhã, Portugal

关键词: <;    i>;    In Vitro<;    /i>;    Antioxidant Activity and Total Phenolic Content of Root Extracts from <;    i>;    Phragmanthera glaucocarpa<;    /i>;    (Peyr.) Balle;    Phenolic Compounds;    DPPH;    ABTS;    Peroxide Value;    Folin-Ciocalteu;    Phragmanthera glaucocarpa;    articles;    62238;   

DOI  :  10.5812/jjnpp.62238

学科分类：药理学

来源: Jundishapur Journal of Natural Pharmaceutical Products

【 摘 要 】

Africa has a rich diversity of plant species, which can be considered important sources of new chemical compounds that can be used in the development of novel therapeutic drugs.The present study aimed at investigating the antioxidant activity and total phenolic content of extracts obtained from the roots of Phragmanthera glaucocarpa (Peyr.) Balle, a plant used in Angola folk medicine.The prepared extracts were tested for their antioxidant activity using ABTS (2,2’-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid), DPPH (1’,1’-diphenyl-2-picrylhydrazyl), and peroxide value methods. Total phenolics were evaluated by the Folin-Ciocalteu method.For ABTS, the best EC50 result was obtained with the ethyl acetate fraction (1.06 ± 0.30 mg/mL). For the DPPH method, the methanol extract and aqueous fraction presented the best EC50 results, 0.10 ± 0.01 mg/mL and 0.10 ± 0.00 mg/mL, respectively. The ethyl acetate fraction had the highest phenolic content (280.42 ± 0.15 mg G.A.E. /g of dry extract). The best result for the peroxide reduction value on the eighth day of sunflower oil oxidation was obtained for the aqueous fraction, with 131.40 ± 1.05 meq O2/g of lipid sample.The results of the present study demonstrate that the roots of the medicinal plant Phragmanthera glaucocarpa (Peyr.) Balle represent a promising source of natural phenolic compounds with antioxidant activity.

【 授权许可】

CC BY-NC