Paracrine mechanisms in early differentiation of human pluripotent stem cells: Insights from a mathematical model - ScienceDirect

Stem Cell Research

Erika Gaspari^1,3¹  Diana Robles-Diaz^2²  Annika Franke^2³ 

[1] Institute for Medical Informatics and Biometry, Carl Gustav Carus Faculty of Medicine, TU Dresden, Dresden, Germany^1;Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, the Netherlands^3;Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Cluster of Excellence, Hannover Medical School, Germany^2

关键词: Human pluripotent stem cells;    Paracrine effects;    Differentiation;    Primitive streak;    Mathematical modeling;   

DOI  :  10.1016/j.scr.2018.07.025

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

With their capability to self-renew and differentiate into derivatives of all three germ layers, human pluripotent stem cells (hPSCs) offer a unique model to study aspects of human development in vitro. Directed differentiation towards mesendodermal lineages is a complex process, involving transition through a primitive streak (PS)-like stage. We have recently shown PS-like patterning from hPSCs into definitive endoderm, cardiac as well as presomitic mesoderm by only modulating the bulk cell density and the concentration of the GSK3 inhibitor CHIR99021, a potent activator of the WNT pathway. The patterning process is modulated by a complex paracrine network, whose identity and mechanistic consequences are poorly understood.

【 授权许可】

Unknown