Stem Cell Research | |
Paracrine mechanisms in early differentiation of human pluripotent stem cells: Insights from a mathematical model - ScienceDirect | |
Erika Gaspari^1,31  Diana Robles-Diaz^22  Annika Franke^23  | |
[1] Institute for Medical Informatics and Biometry, Carl Gustav Carus Faculty of Medicine, TU Dresden, Dresden, Germany^1;Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, the Netherlands^3;Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Cluster of Excellence, Hannover Medical School, Germany^2 | |
关键词: Human pluripotent stem cells; Paracrine effects; Differentiation; Primitive streak; Mathematical modeling; | |
DOI : 10.1016/j.scr.2018.07.025 | |
学科分类:生物科学(综合) | |
来源: Elsevier | |
【 摘 要 】
With their capability to self-renew and differentiate into derivatives of all three germ layers, human pluripotent stem cells (hPSCs) offer a unique model to study aspects of human development in vitro. Directed differentiation towards mesendodermal lineages is a complex process, involving transition through a primitive streak (PS)-like stage. We have recently shown PS-like patterning from hPSCs into definitive endoderm, cardiac as well as presomitic mesoderm by only modulating the bulk cell density and the concentration of the GSK3 inhibitor CHIR99021, a potent activator of the WNT pathway. The patterning process is modulated by a complex paracrine network, whose identity and mechanistic consequences are poorly understood.
【 授权许可】
Unknown
【 预 览 】
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